Fabrication of L-lactate biosensor based on redox species mediated lactate oxidase using micro-device
نویسنده
چکیده
Original article Lactate biosensor Lactate Oxidase TGA, K Fe(CN) Micro-device Cyclic voltammetry 3 6
منابع مشابه
Electrochemical l-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lac...
متن کاملAmperometric bienzymatic biosensor for L-lactate analysis in wine and beer samples.
A novel amperometric bienzymatic biosensor has been developed based on the incorporation of Lactate Oxidase (LOx) and Horseradish Peroxidase (HRP) into a carbon nanotube/polysulfone membrane by the phase inversion technique onto screen-printed electrodes (SPEs). In order to improve the sensitivity and reduce the working potential, experimental conditions have been optimized and ferrocene has al...
متن کاملImmobilizing enzymes onto electrode arrays by hydrogel photolithography to fabricate multi-analyte electrochemical biosensors.
This paper describes a biomaterial microfabrication approach for interfacing functional biomolecules (enzymes) with electrode arrays. Poly (ethylene glycol) (PEG) hydrogel photopatterning was employed to integrate gold electrode arrays with the enzymes glucose oxidase (GOX) and lactate oxidase (LOX). In this process, PEG diacrylate (DA)-based prepolymer containing enzyme molecules as well as re...
متن کاملAmperometric biosensor for lactate analysis in wine and must during fermentation
A lactate oxidase-based amperometric biosensor is designed for lactate determination. Two methods of lactate oxidase immobilization on the surface of commercial SensLab platinum printing electrodes are compared. The sensor with lactate oxidase immobilized by physical adsorption in Resydrol polymer is shown to have both narrower dynamic range (0.004– 0.5 mМ lactate) and higher sensitivity (320 n...
متن کاملThe Role of Metal Oxide Layers in the Sensitivity of Lactate Biosensors Subjected to Oxygen-Limited Conditions
Amperometric lactate biosensors are used to detect lactate concentration in blood and tissues, which is integral in identifying cyanide poisoning, septic shock, and athletic condition. The construction of lactate biosensors with high sensitivity, selectivity, and stability is imperative to diagnose and determine these medical conditions. Lactate detection is currently limited to oxygen-rich env...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010